Format

Send to

Choose Destination
J Clin Microbiol. 2015 Feb;53(2):382-8. doi: 10.1128/JCM.02033-14. Epub 2014 Nov 19.

Detection of chikungunya virus antigen by a novel rapid immunochromatographic test.

Author information

1
Mahidol-Osaka Center for Infectious Diseases (MOCID), Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan tamaoka@biken.osaka-u.ac.jp.
2
Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
3
Mahidol-Osaka Center for Infectious Diseases (MOCID), Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Center for Vaccine Development, Mahidol University, Nakhon Pathom, Thailand.
4
Mahidol-Osaka Center for Infectious Diseases (MOCID), Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
5
Center for Vaccine Development, Mahidol University, Nakhon Pathom, Thailand.
6
Division of Infectious Disease, Department of Internal Medicine, Faculty of Medicine, Prince of Songkla University, Hat Yai, Thailand.
7
Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
8
Institute Pasteur du Laos, Vientiane, Lao People's Democratic Republic.
9
Indonesia-Japan Collaboration Research Center for Emerging and Re-emerging Infectious Diseases, Institute of Tropical Diseases, Airlangga University, Surabaya, Indonesia.
10
Indonesia-Japan Collaboration Research Center for Emerging and Re-emerging Infectious Diseases, Institute of Tropical Diseases, Airlangga University, Surabaya, Indonesia Center for Infectious Diseases, Kobe University Graduate School of Medicine, Hyogo, Japan.
11
Arboviruses and Viral Hemorrhagic Fever Unit, Institute Pasteur de Dakar, Dakar, Senegal.
12
Alfresa Pharma Corporation, Osaka, Japan.
13
Alfresa Pharma Corporation, Osaka, Japan Arkray Incorporated, Kyoto, Japan.
14
Center of Excellence for Antibody Research, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

Abstract

Chikungunya fever is a mosquito-borne disease of key public health importance in tropical and subtropical countries. Although severe joint pain is the most distinguishing feature of chikungunya fever, diagnosis remains difficult because the symptoms of chikungunya fever are shared by many pathogens, including dengue fever. The present study aimed to develop a new immunochromatographic diagnosis test for the detection of chikungunya virus antigen in serum. Mice were immunized with isolates from patients with Thai chikungunya fever, East/Central/South African genotype, to produce mouse monoclonal antibodies against chikungunya virus. Using these monoclonal antibodies, a new diagnostic test was developed and evaluated for the detection of chikungunya virus. The newly developed diagnostic test reacted with not only the East/Central/South African genotype but also with the Asian and West African genotypes of chikungunya virus. Testing of sera from patients suspected to have chikungunya fever in Thailand (n = 50), Laos (n = 54), Indonesia (n = 2), and Senegal (n = 6) revealed sensitivity, specificity, and real-time PCR (RT-PCR) agreement values of 89.4%, 94.4%, and 91.1%, respectively. In our study using serial samples, a new diagnostic test showed high agreement with the RT-PCR within the first 5 days after onset. A rapid diagnostic test was developed using mouse monoclonal antibodies that react with chikungunya virus envelope proteins. The diagnostic accuracy of our test is clinically acceptable for chikungunya fever in the acute phase.

PMID:
25411170
PMCID:
PMC4298496
DOI:
10.1128/JCM.02033-14
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center