A monoclonal antibody to CD11c antigen inhibits the production of superoxide anion induced by concanavalin A in PMA-differentiated U-937 cells

Immunol Lett. 1989 Feb;20(3):193-7. doi: 10.1016/0165-2478(89)90079-5.

Abstract

We have examined the effect of different monoclonal antibodies (MAb) to the CD11/CD18 family of surface antigens on the production of superoxide anion by phorbol myristate acetate (PMA)-differentiated U-937 cells upon stimulation with concanavalin A (Con A). The anti-CD11c MAb HC1/1 displayed a significant inhibition, whereas MAb to the other members of the CD11 family did not show a relevant inhibitory effect. These findings suggest that the CD11c antigen is involved in the triggering of the respiratory burst response by mononuclear phagocytic cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal*
  • Antigens, Differentiation / metabolism*
  • Antigens, Surface
  • Calcium / metabolism
  • Cell Line
  • Concanavalin A / pharmacology*
  • Integrin alphaXbeta2
  • Superoxides / biosynthesis*
  • Tetradecanoylphorbol Acetate / pharmacology*

Substances

  • Antibodies, Monoclonal
  • Antigens, Differentiation
  • Antigens, Surface
  • Integrin alphaXbeta2
  • Concanavalin A
  • Superoxides
  • Tetradecanoylphorbol Acetate
  • Calcium