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PLoS One. 2014 Nov 19;9(11):e113078. doi: 10.1371/journal.pone.0113078. eCollection 2014.

Frequency and fitness consequences of bacteriophage φ6 host range mutations.

Author information

1
Biology Department, Queens College of the City University of New York, New York, New York, United States of America; The Graduate Center of the City University of New York, New York, New York, United States of America.
2
Biology Department, Queens College of the City University of New York, New York, New York, United States of America.

Abstract

Viruses readily mutate and gain the ability to infect novel hosts, but few data are available regarding the number of possible host range-expanding mutations allowing infection of any given novel host, and the fitness consequences of these mutations on original and novel hosts. To gain insight into the process of host range expansion, we isolated and sequenced 69 independent mutants of the dsRNA bacteriophage Φ6 able to infect the novel host, Pseudomonas pseudoalcaligenes. In total, we found at least 17 unique suites of mutations among these 69 mutants. We assayed fitness for 13 of 17 mutant genotypes on P. pseudoalcaligenes and the standard laboratory host, P. phaseolicola. Mutants exhibited significantly lower fitnesses on P. pseudoalcaligenes compared to P. phaseolicola. Furthermore, 12 of the 13 assayed mutants showed reduced fitness on P. phaseolicola compared to wildtype Φ6, confirming the prevalence of antagonistic pleiotropy during host range expansion. Further experiments revealed that the mechanistic basis of these fitness differences was likely variation in host attachment ability. In addition, using computational protein modeling, we show that host-range expanding mutations occurred in hotspots on the surface of the phage's host attachment protein opposite a putative hydrophobic anchoring domain.

PMID:
25409341
PMCID:
PMC4237377
DOI:
10.1371/journal.pone.0113078
[Indexed for MEDLINE]
Free PMC Article

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