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J Vis Exp. 2014 Nov 9;(93):e51949. doi: 10.3791/51949.

Confocal time lapse imaging as an efficient method for the cytocompatibility evaluation of dental composites.

Author information

1
Laboratoire des Multimatériaux et Interfaces, UMR CNRS 5615, Université Lyon1; nina.attik@univ-lyon1.fr.
2
Laboratoire des Multimatériaux et Interfaces, UMR CNRS 5615, Université Lyon1; UFR d'Odontologie, Université Lyon1; Service de Consultations et de Traitements Dentaires, Hospices Civils de Lyon.
3
Laboratoire des Multimatériaux et Interfaces, UMR CNRS 5615, Université Lyon1; UFR d'Odontologie, Université Paris Diderot; Service d'Odontologie, APHP, Hôpital Rothschild.

Abstract

It is generally accepted that in vitro cell material interaction is a useful criterion in the evaluation of dental material biocompatibility. The objective of this study was to use 3D CLSM time lapse confocal imaging to assess the in vitro biocompatibility of dental composites. This method provides an accurate and sensitive indication of viable cell rate in contact with dental composite extracts. The ELS extra low shrinkage, a dental composite used for direct restoration, has been taken as example. In vitro assessment was performed on cultured primary human gingival fibroblast cells using Live/Dead staining. Images were obtained with the FV10i confocal biological inverted system and analyzed with the FV10-ASW 3.1 Software. Image analysis showed a very slight cytotoxicity in the presence of the tested composite after 5 hours of time lapse. A slight decrease of cell viability was shown in contact with the tested composite extracts compared to control cells. The findings highlighted the use of 3D CLSM time lapse imaging as a sensitive method to qualitatively and quantitatively evaluate the biocompatibility behavior of dental composites.

PMID:
25406737
PMCID:
PMC4353434
DOI:
10.3791/51949
[Indexed for MEDLINE]
Free PMC Article

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