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Molecules. 2014 Nov 14;19(11):18721-32. doi: 10.3390/molecules191118721.

Methanolic extracts of Solieria robusta inhibits proliferation of oral cancer Ca9-22 cells via apoptosis and oxidative stress.

Author information

1
Department of Dentistry, Ten Chan General Hospital, Chung-Li 32043, Taiwan. theericyen@yahoo.com.
2
Laboratory for Translational Oncology and Personalized Medicine, Rashid Latif Medical College, Lahore 54000, Pakistan. ammadfarooqi@rlmclahore.com.
3
Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. sherry30126@yahoo.com.tw.
4
Department of Botany, Government College University, Lahore, Katchery Road Lahore 54000, Pakistan. drghazala71@yahoo.com.
5
Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. reyata@kmu.edu.tw.
6
Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan. cywu@mail.nsysu.edu.tw.
7
Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. mifeho@kmu.edu.tw.
8
Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. changhw@kmu.edu.tw.

Abstract

Many red algae-derived natural products are known to have anticancer effects. The biological functions of the red alga Solieria robusta from the Karachi coast (Pakistan) remain unclear. Here, we prepared a methanolic extracts of S. robusta (MESR) to examine its possible anti-oral cancer effects and the corresponding mechanism of action. Cell viability of MESR-incubated oral cancer Ca9-22 cells was dose-responsively decreased (p<0.001). According to a propidium iodide (PI)-based assay the cell cycle distribution was dramatically changed, especially for subG1 accumulation. Annexin V/PI assay of apoptosis using flow cytometry also showed that MESR-incubated Ca9-22 cells were dose-responsively increased (p<0.001). For evaluation of oxidative stress in MESR-incubated Ca9-22 cells, we found that reactive oxygen species (ROS) were overexpressed dose- and time-responsively and mitochondrial depolarization was also increased (p<0.001). Taken together, MESR showed inhibitory effects on oral cancer proliferation coupled with apoptosis and oxidative stress.

PMID:
25405289
PMCID:
PMC6271418
DOI:
10.3390/molecules191118721
[Indexed for MEDLINE]
Free PMC Article

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