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Eur J Haematol. 2015 Sep;95(3):239-43. doi: 10.1111/ejh.12486. Epub 2015 Jan 7.

Interleukin-10 inhibits autonomous myelopoiesis in patients with myelofibrosis.

Author information

1
5th Department of Internal Medicine - Oncology/Hematology, Hospital Hietzing, Vienna, Austria.
2
Ludwig Boltzmann Institute for Clinical Oncology, Vienna, Austria.
3
Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria.
4
Department of Internal Medicine I, St-Josef-Krankenhaus, Vienna, Austria.
5
Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Vienna, Austria.

Abstract

The spontaneous formation of colony-forming units granulocyte/macrophage (CFU-GM) in semisolid cultures has been shown to be due to the endogenous release of cytokines and/or to the hypersensitivity of cells against growth factors. We have reported that increased autonomous CFU-GM growth is an in vitro characteristic of myelofibrosis (MF) which may reflect aberrant hematopoiesis in vivo. Because of its cytokine synthesis-inhibiting action, we speculated that interleukin-10 (IL-10) may inhibit pathological overproduction of myeloid cells in MF by suppression of autonomous myelopoiesis. In this study, IL-10 significantly inhibited autonomous CFU-GM formation in vitro from peripheral blood mononuclear cells (PB MNC) in 10 of 11 patients with MF tested. In all patients, there was a mean inhibition of 69% ranging from 35% to 100%. Suppression of autonomous CFU-GM formation by IL-10 was dose dependent and reversible by the addition of anti-IL-10 antibodies. Our results indicate that IL-10 is a potentially useful molecule to affect aberrant myelopoiesis in patients with MF.

KEYWORDS:

interleukin-10; myelofibrosis; spontaneous CFU-GM

PMID:
25404526
DOI:
10.1111/ejh.12486
[Indexed for MEDLINE]

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