Format

Send to

Choose Destination
Nat Neurosci. 2014 Dec;17(12):1816-24. doi: 10.1038/nn.3866. Epub 2014 Nov 17.

Simultaneous cellular-resolution optical perturbation and imaging of place cell firing fields.

Author information

1
1] Princeton Neuroscience Institute, Princeton University, Princeton, New Jersey, USA. [2] Bezos Center for Neural Circuit Dynamics, Princeton University, Princeton, New Jersey, USA. [3] Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA. [4] Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA.
2
1] Department of Bioengineering, Stanford University, Stanford, California, USA. [2] CNC Program, Stanford University, Stanford, California, USA. [3] Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, California, USA. [4] Howard Hughes Medical Institute, Stanford University, Stanford, California, USA.

Abstract

Linking neural microcircuit function to emergent properties of the mammalian brain requires fine-scale manipulation and measurement of neural activity during behavior, where each neuron's coding and dynamics can be characterized. We developed an optical method for simultaneous cellular-resolution stimulation and large-scale recording of neuronal activity in behaving mice. Dual-wavelength two-photon excitation allowed largely independent functional imaging with a green fluorescent calcium sensor (GCaMP3, λ = 920 ± 6 nm) and single-neuron photostimulation with a red-shifted optogenetic probe (C1V1, λ = 1,064 ± 6 nm) in neurons coexpressing the two proteins. We manipulated task-modulated activity in individual hippocampal CA1 place cells during spatial navigation in a virtual reality environment, mimicking natural place-field activity, or 'biasing', to reveal subthreshold dynamics. Notably, manipulating single place-cell activity also affected activity in small groups of other place cells that were active around the same time in the task, suggesting a functional role for local place cell interactions in shaping firing fields.

PMID:
25402854
PMCID:
PMC4459599
DOI:
10.1038/nn.3866
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center