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Cell Biochem Biophys. 2015 Apr;71(3):1525-36. doi: 10.1007/s12013-014-0376-8.

Acrolein-Induced Oxidative Stress and Cell Death Exhibiting Features of Apoptosis in the Yeast Saccharomyces cerevisiae Deficient in SOD1.

Author information

1
Department of Biochemistry and Cell Biology, University of Rzeszow, Zelwerowicza 4, 35-601, Rzeszow, Poland.
2
Department of Biochemistry and Cell Biology, University of Rzeszow, Zelwerowicza 4, 35-601, Rzeszow, Poland. skoziol@univ.rzeszow.pl.

Abstract

The yeast Saccharomyces cerevisiae is a useful eukaryotic model to study the toxicity of acrolein, an important environmental toxin and endogenous product of lipid peroxidation. The study was aimed at elucidation of the cytotoxic effect of acrolein on the yeast deficient in SOD1, Cu, Zn-superoxide dismutase which is hypersensitive to aldehydes. Acrolein generated within the cell from its precursor allyl alcohol caused growth arrest and cell death of the yeast cells. The growth inhibition involved an increase in production of reactive oxygen species and high level of protein carbonylation. DNA condensation and fragmentation, exposition of phosphatidylserine at the cell surface as well as decreased dynamic of actin microfilaments and mitochondria disintegration point to the induction of apoptotic-type cell death besides necrotic cell death.

KEYWORDS:

Acrolein; Allyl alcohol; Apoptosis; Cell death; Oxidative stress; Superoxide dismutase; Yeast

PMID:
25395196
PMCID:
PMC4449388
DOI:
10.1007/s12013-014-0376-8
[Indexed for MEDLINE]
Free PMC Article

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