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Exp Dermatol. 2015 Feb;24(2):157-9. doi: 10.1111/exd.12594.

Comparative analysis of colorimetric staining in skin using open-source software.

Author information

1
Department of Radiation Oncology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Abstract

Colorimetric staining techniques such as immunohistochemistry (IHC), immunofluorescence (IF) and histochemistry (HC) provide useful information regarding the localization and relative amount of a molecule/substance in skin. We have developed a novel, straightforward method to assess colorimetric staining by combining features from two open-source software programs. As a proof of principle, we demonstrate the utility of this approach by analysing changes in skin melanin deposition during the radiation-induced tanning response of Yucatan mini-pigs. This method includes a visualization step to validate the accuracy of colour selection before quantitation to ensure accuracy. The data show that this method is robust and will provide a means to obtain accurate comparative analyses of staining in IHC/IF/HC samples.

KEYWORDS:

analysis; histochemistry; immunofluorescence; immunohistochemistry; method

PMID:
25393687
PMCID:
PMC4479192
DOI:
10.1111/exd.12594
[Indexed for MEDLINE]
Free PMC Article

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