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Oncogene. 1989 Feb;4(2):189-93.

Activated N-ras gene was found in human hepatoma tissue but only in a small fraction of the tumor cells.

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  • 1Department of Gene Research, Cancer Institute, Tokyo, Japan.


Activated N-ras gene was isolated from human hepatoma tissue by DNA transfection assay coupled with the neomycin selection method and molecular cloning and a point mutation in the codon 61 (CAA----AAA) was noted. However, examination of the proportion of the mutated N-ras gene in the tumor part by molecular cloning and by hybridization using synthetic oligonucleotide probes indicated that the mutated gene occurred with very low frequency. The activated N-ras gene appears located only in a small fraction of the tumor cells. The experimental data indicate activation of this gene as possibly not the major cause of carcinoma, but rather a manifestation of tumor heterogeneity.

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