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AIDS. 2015 Jan 2;29(1):43-51. doi: 10.1097/QAD.0000000000000511.

Gut epithelial barrier and systemic inflammation during chronic HIV infection.

Author information

1
aDivision of Gastroenterology, Department of Medicine, University of California, San Francisco (UCSF), San Francisco, California bAIDS and Cancer Virus Program, Leidos Biomedical Research Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland cDivision of Experimental Medicine, Department of Medicine, UCSF, San Francisco, California dDivision of Gastroenterology, Department of Medicine, University of Washington, Washington eDepartment of Epidemiology and Biostatistics fHIV/AIDS Division, Department of Medicine, UCSF, San Francisco, California, USA.

Abstract

OBJECTIVE:

Microbial translocation and innate immune action characterize HIV infection. Continued gut mucosal dysfunction during treatment and its relationship to CD4 T-cell recovery has not been well described.

DESIGN:

A cross-sectional study was performed of antiretroviral therapy (ART)-suppressed (immunologic responders with CD4 > 500 cells/μl and immunologic nonresponders with CD4 < 350 cells/μl), untreated HIV-infected, and seronegative participants consenting to gut biopsies and a blood draw.

METHODS:

Neutrophil infiltration as a surrogate response to epithelial breach, colorectal epithelial proliferation as a measure of repair, and mucosal apoptosis by immunohistochemistry were determined in gut biopsies. Plasma markers of monocyte activation (sCD14), immune activation (interleukin-6), and indoleamine 2,3-dioxygenase-1 activity (plasma kynurenine/tryptophanratio) were concurrently measured.

RESULTS:

Each HIV-infected group had greater neutrophil infiltration than controls. Similarly, untreated HIV-infected participants and ART-suppressed immunologic responders had increased epithelial proliferation compared with controls, but immunologic nonresponders had no appreciable increase in epithelial proliferation despite elevated neutrophil infiltration. The CD4 T-cell count was positively correlated with epithelial proliferation and was modestly negatively correlated with neutrophil infiltration in ART-suppressed patients. Epithelial proliferation was inversely correlated with mucosal apoptosis, and apoptosis was linked to plasma sCD14 and modestly to kynurenine/tryptophan ratio.

CONCLUSIONS:

Neutrophil infiltration and mucosal apoptosis remain abnormally high despite ART. Epithelial proliferation increases in HIV, but may be impaired in immunologic nonresponders. Whether mucosal apoptosis is a cause or consequence of epithelial proliferative defects is unclear, but appears to be associated with systemic inflammation. The impact of ART and interventions targeting the gut epithelial barrier in treated HIV infection warrant further investigation.

PMID:
25387317
PMCID:
PMC4444362
DOI:
10.1097/QAD.0000000000000511
[Indexed for MEDLINE]
Free PMC Article

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