Format

Send to

Choose Destination
Proc Natl Acad Sci U S A. 2014 Nov 25;111(47):16766-71. doi: 10.1073/pnas.1416121111. Epub 2014 Nov 10.

TSG-6 as a biomarker to predict efficacy of human mesenchymal stem/progenitor cells (hMSCs) in modulating sterile inflammation in vivo.

Author information

1
Institute for Regenerative Medicine, Texas A&M Health Science Center College of Medicine at Scott and White, Temple, TX 76502; and rlee@medicine.tamhsc.edu Prockop@medicine.tamhsc.edu.
2
Institute for Regenerative Medicine, Texas A&M Health Science Center College of Medicine at Scott and White, Temple, TX 76502; and.
3
Department of Ophthalmology, Seoul National University Hospital, Jongno-gu, Seoul 110-744, Korea.

Abstract

Human mesenchymal stem/progenitor cells (hMSCs) from bone marrow and other tissues are currently being administered to large numbers of patients even though there are no biomarkers that accurately predict their efficacy in vivo. Using a mouse model of chemical injury of the cornea, we found that bone-marrow-derived hMSCs isolated from different donors varied widely in their efficacy in modulating sterile inflammation. Importantly, RT-PCR assays of hMSCs for the inflammation-modulating protein TSG-6 expressed by the TNFα-stimulated gene 6 (TSG-6 or TNFAIP6) predicted their efficacy in sterile inflammation models for corneal injury, sterile peritonitis, and bleomycin-induced lung injury. In contrast, the levels of TSG-6 mRNA were negatively correlated with their potential for osteogenic differentiation in vitro and poorly correlated with other criteria for evaluating hMSCs. Also, a survey of a small cohort suggested that hMSCs from female donors compared with male donors more effectively suppressed sterile inflammation, expressed higher levels of TSG-6, and had slightly less osteogenic potential.

KEYWORDS:

MPO; gender; myeloperoxidase; neutrophils

PMID:
25385603
PMCID:
PMC4250139
DOI:
10.1073/pnas.1416121111
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center