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Lab Chip. 2015 Jan 7;15(1):52-6. doi: 10.1039/c4lc01040j.

Rapid modulation of droplet composition with pincer microvalves.

Author information

1
Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California San Francisco, San Francisco, California, USA. adam.abate@ucsf.edu.

Abstract

Single-layer membrane valves are simple to fabricate and to integrate into microfluidic devices. However, due to their rectangular flow channel geometry, they do not fully seal. Here, we show that liquid flow can be reduced by over 3 orders of magnitude, enabling the contents of forming droplets to be dynamically modulated. We use this precision control to perform combinatorial DNA synthesis in the droplets.

PMID:
25385230
PMCID:
PMC5531040
DOI:
10.1039/c4lc01040j
[Indexed for MEDLINE]
Free PMC Article

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