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J Infect Dis. 2015 Apr 15;211(8):1334-41. doi: 10.1093/infdis/jiu605. Epub 2014 Nov 7.

Hyperimmune bovine colostrum as a novel therapy to combat Clostridium difficile infection.

Author information

1
Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine, Tufts University, North Grafton, Massachusetts.
2
Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine, Tufts University, North Grafton, Massachusetts Department of Animal Resources Science, Dankook University, Cheonan, Choongnam, Republic of Korea.

Abstract

BACKGROUND:

Clostridium difficile is a primary cause of antibiotic-associated diarrhea that typically develops when gut microbiota is altered. Conventional treatment for C. difficile infection (CDI) is additional antimicrobial administration, which further disrupts normal intestinal microbiota, often resulting in poor treatment outcomes.

METHODS:

A pregnant dairy cow was repeatedly immunized with recombinant mutants of toxins A and B produced by C. difficile, and the resultant hyperimmune bovine colostrum (HBC) was evaluated for therapeutic efficacy in gnotobiotic piglets with diarrhea due to CDI. Control piglets received nonimmune colostrum. To determine the impact of HBC on gut microbiota, 1 of 2 groups of piglets transplanted with normal human gut microbiota was treated with HBC.

RESULTS:

Nonimmune colostrum-treated piglets developed moderate to severe diarrhea and colitis. In contrast, HBC-treated piglets had mild or no diarrhea and mild or no colitis. Lyophilization had no detectable impact on HBC efficacy. HBC had no discernible effect on the composition of normal human gut microbiota in the porcine intestinal tract.

CONCLUSIONS:

HBC provides an oral, cost-effective, and safe alternative to antibiotic therapy for CDI. By preserving intestinal microbiota, HBC may be more efficacious than antibiotics. Additional studies are warranted to establish HBC as a viable immunotherapeutic agent for human use against CDI.

KEYWORDS:

Clostridium difficile; hyperimmune bovine colostrum; immunotherapeutic; intestinal microbiota; intestinal microflora

PMID:
25381448
PMCID:
PMC4447838
DOI:
10.1093/infdis/jiu605
[Indexed for MEDLINE]
Free PMC Article

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