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Science. 2014 Nov 7;346(6210):755-9. doi: 10.1126/science.1257147.

Enteric bacteria promote human and mouse norovirus infection of B cells.

Author information

1
Department of Molecular Genetics and Microbiology, Emerging Pathogens Institute, College of Medicine, University of Florida, Gainesville, FL, USA.
2
Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL, USA. Department of Periodontology, College of Dentistry, University of Florida, Gainesville, FL, USA.
3
Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA.
4
Department of Medicine, Division of Infectious Diseases, University of Florida, Gainesville, FL, USA.
5
Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
6
Department of Molecular Genetics and Microbiology, Emerging Pathogens Institute, College of Medicine, University of Florida, Gainesville, FL, USA. skarst@ufl.edu.

Abstract

The cell tropism of human noroviruses and the development of an in vitro infection model remain elusive. Although susceptibility to individual human norovirus strains correlates with an individual's histo-blood group antigen (HBGA) profile, the biological basis of this restriction is unknown. We demonstrate that human and mouse noroviruses infected B cells in vitro and likely in vivo. Human norovirus infection of B cells required the presence of HBGA-expressing enteric bacteria. Furthermore, mouse norovirus replication was reduced in vivo when the intestinal microbiota was depleted by means of oral antibiotic administration. Thus, we have identified B cells as a cellular target of noroviruses and enteric bacteria as a stimulatory factor for norovirus infection, leading to the development of an in vitro infection model for human noroviruses.

Comment in

PMID:
25378626
PMCID:
PMC4401463
DOI:
10.1126/science.1257147
[Indexed for MEDLINE]
Free PMC Article

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