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J Clin Microbiol. 2015 Jan;53(1):191-200. doi: 10.1128/JCM.02589-14. Epub 2014 Nov 5.

Whole-genome phylogenomic heterogeneity of Neisseria gonorrhoeae isolates with decreased cephalosporin susceptibility collected in Canada between 1989 and 2013.

Author information

1
Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada Walter.Demczuk@phac-aspc.gc.ca.
2
Alberta Health Services, Calgary, Alberta, Canada.
3
Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
4
Science Technology Cores and Services Division, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada, and Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada.
5
Public Health Ontario Laboratories, Toronto, Ontario, Canada.
6
British Columbia Public Health Microbiology and Reference Laboratory, British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada.
7
Laboratoire de Santé Publique du Québec, Sainte-Anne-de-Bellevue, Québec, Canada.
8
Provincial Laboratory for Public Health, Edmonton, Alberta, Canada.
9
Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada.
10
Queen Elizabeth II Health Sciences Centre, Halifax, Nova Scotia, Canada.
11
Dr. G.L. Dumont Hospital, Moncton, New Brunswick, Canada.
12
Cadham Provincial Laboratory, Winnipeg, Manitoba, Canada.
13
Centre for Communicable Diseases and Infection Control, Public Health Agency of Canada, Ottawa, Ontario, Canada.

Abstract

A large-scale, whole-genome comparison of Canadian Neisseria gonorrhoeae isolates with high-level cephalosporin MICs was used to demonstrate a genomic epidemiology approach to investigate strain relatedness and dynamics. Although current typing methods have been very successful in tracing short-chain transmission of gonorrheal disease, investigating the temporal evolutionary relationships and geographical dissemination of highly clonal lineages requires enhanced resolution only available through whole-genome sequencing (WGS). Phylogenomic cluster analysis grouped 169 Canadian strains into 12 distinct clades. While some N. gonorrhoeae multiantigen sequence types (NG-MAST) agreed with specific phylogenomic clades or subclades, other sequence types (ST) and closely related groups of ST were widely distributed among clades. Decreased susceptibility to extended-spectrum cephalosporins (ESC-DS) emerged among a group of diverse strains in Canada during the 1990s with a variety of nonmosaic penA alleles, followed in 2000/2001 with the penA mosaic X allele and then in 2007 with ST1407 strains with the penA mosaic XXXIV allele. Five genetically distinct ESC-DS lineages were associated with penA mosaic X, XXXV, and XXXIV alleles and nonmosaic XII and XIII alleles. ESC-DS with coresistance to azithromycin was observed in 5 strains with 23S rRNA C2599T or A2143G mutations. As the costs associated with WGS decline and analysis tools are streamlined, WGS can provide a more thorough understanding of strain dynamics, facilitate epidemiological studies to better resolve social networks, and improve surveillance to optimize treatment for gonorrheal infections.

PMID:
25378573
PMCID:
PMC4290921
DOI:
10.1128/JCM.02589-14
[Indexed for MEDLINE]
Free PMC Article

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