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J Appl Microbiol. 2015 Feb;118(2):506-14. doi: 10.1111/jam.12686. Epub 2014 Dec 22.

Cell-bound exopolysaccharides of Lactobacillus brevis KB290 enhance cytotoxic activity of mouse splenocytes.

Author information

1
Nature & Wellness Research Department, Research Institute, Kagome Co. Ltd., Nasushiobara, Tochigi, Japan.

Abstract

AIMS:

This study aimed to identify the main active component of Lactobacillus brevis KB290 (KB290) that is responsible for enhanced cell-mediated cytotoxic activity of mouse splenocytes Live KB290, a probiotic strain derived from a Japanese traditional pickle, was previously reported to modulate innate immune responses as affecting on cell-mediated cytotoxic activity of mouse splenocytes.

METHODS AND RESULTS:

We used live KB290, heat-killed KB290, a derivative strain (Lact. brevis KB392) with different amounts of cell-bound exopolysaccharide (EPS-b), and a crude extract of EPS-b from KB290 cell surface. Female BALB/c mice were fed a diet containing 10(10) CFU live KB290, 10(10) CFU live KB392, 15 mg heat-killed KB290 or 600 μg crude extract of EPS-b for 1 day. Live KB290 (P < 0.01), heat-killed KB290 (P < 0.05) and crude EPS-b at 600 μg (P < 0.05) per mouse significantly enhanced cytotoxic activity; however, live KB392 had no effect.

CONCLUSIONS:

Both live and heat-killed KB290 and crude EPS-b significantly enhanced cytotoxic activity of mouse splenocytes.

SIGNIFICANCE AND IMPACT OF THE STUDY:

We demonstrated that EPS-b produced by KB290 has a critical role in enhancing cell-mediated cytotoxic activity in mouse spleen.

KEYWORDS:

Lactobacillus brevis KB290; Lactobacillus brevis KB392; cell-bound exopolysaccharides; cell-mediated cytotoxicity; heat-killed KB290

PMID:
25376258
DOI:
10.1111/jam.12686
[Indexed for MEDLINE]
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