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Regen Med. 2014;9(5):609-19. doi: 10.2217/rme.14.33.

First transplantation of isolated murine follicles in alginate.

Author information

1
Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, 1200 Brussels, Belgium.

Abstract

AIM:

Our aim is to develop an artificial ovary allowing survival and growth of isolated follicles and ovarian cells, to restore fertility in women diagnosed with pathologies at high risk of ovarian involvement.

MATERIALS & METHODS:

For this, alginate beads containing isolated preantral follicles and ovarian cells were autografted to immunocompetent mice. One week after grafting, the beads were invaded by proliferating murine cells (12.1%) and capillaries.

RESULTS:

The recovery rate of follicles per graft ranged from 0% to 35.5%. Of the analyzed follicles, 77% were Ki67-positive and 81%, TUNEL-negative. Three antral follicles were also identified, evidencing their ability to grow in the matrix.

CONCLUSION:

Our results suggest that an artificial ovary is now conceivable, opening new perspectives to restore fertility in women.

KEYWORDS:

alginate; artificial ovary; cancer; degradation; isolated preantral follicles; ovarian cells

PMID:
25372078
DOI:
10.2217/rme.14.33
[Indexed for MEDLINE]

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