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Antimicrob Agents Chemother. 2015 Jan;59(1):427-36. doi: 10.1128/AAC.03954-14. Epub 2014 Nov 3.

The resistome of Pseudomonas aeruginosa in relationship to phenotypic susceptibility.

Author information

1
Infection Innovative Medicines Unit, AstraZeneca R&D Boston, Waltham, Massachusetts, USA veronica.kos@astrazeneca.com.
2
Infectious Diseases Research Center, Laval University, Quebec, Quebec, Canada.
3
Infection Innovative Medicines Unit, AstraZeneca R&D Boston, Waltham, Massachusetts, USA.

Abstract

Many clinical isolates of Pseudomonas aeruginosa cause infections that are difficult to eradicate due to their resistance to a wide variety of antibiotics. Key genetic determinants of resistance were identified through genome sequences of 390 clinical isolates of P. aeruginosa, obtained from diverse geographic locations collected between 2003 and 2012 and were related to microbiological susceptibility data for meropenem, levofloxacin, and amikacin. β-Lactamases and integron cassette arrangements were enriched in the established multidrug-resistant lineages of sequence types ST111 (predominantly O12) and ST235 (O11). This study demonstrates the utility of next-generation sequencing (NGS) in defining relevant resistance elements and highlights the diversity of resistance determinants within P. aeruginosa. This information is valuable in furthering the design of diagnostics and therapeutics for the treatment of P. aeruginosa infections.

PMID:
25367914
PMCID:
PMC4291382
DOI:
10.1128/AAC.03954-14
[Indexed for MEDLINE]
Free PMC Article

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