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Sleep. 2014 Nov 1;37(11):1757-65. doi: 10.5665/sleep.4166.

Male fertility is reduced by chronic intermittent hypoxia mimicking sleep apnea in mice.

Author information

1
CIBER Enfermedades Respiratorias, Bunyola, Spain ; Laboratori de la Son, Servei de Pneumologia, Hospital Clínic, Universitat de Barcelona, Barcelona, Spain.
2
INIA, Departamento de Reproducción Animal y Conservación de Recursos Zoogenéticos, Madrid.
3
CIBER Enfermedades Respiratorias, Bunyola, Spain ; Laboratori de la Son, Servei de Pneumologia, Hospital Clínic, Universitat de Barcelona, Barcelona, Spain ; Institut Investigacions Biomèdiques August Pi Sunyer, Barcelona, Spain.
4
CIBER Enfermedades Respiratorias, Bunyola, Spain ; Unitat Biofísica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona, Spain ; Institut Bioenginyeria Catalunya, Barcelona, Spain.
5
CIBER Enfermedades Respiratorias, Bunyola, Spain ; Institut Investigacions Biomèdiques August Pi Sunyer, Barcelona, Spain ; Unitat Biofísica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona, Spain.

Abstract

STUDY OBJECTIVES:

Obstructive sleep apnea (OSA) is characterized by intermittent hypoxia and oxidative stress. However, it is unknown whether intermittent hypoxia mimicking OSA modifies male fertility. We tested the hypothesis that male fertility is reduced by chronic intermittent hypoxia mimicking OSA in a mouse model.

DESIGN:

Case-control comparison in a murine model.

SETTING:

University research laboratory.

PARTICIPANTS:

Eighteen F1 (C57BL/6xCBA) male mice.

INTERVENTIONS:

Mice were subjected to a pattern of periodic hypoxia (20 sec at 5% O2 followed by 40 sec of room air) 6 h/day for 60 days or normoxia. After this period, mice performed a mating trial to determine effective fertility by assessing the number of pregnant females and fetuses.

MEASUREMENTS AND RESULTS:

After euthanasia, oxidative stress in testes was assessed by measuring the expression of glutathione peroxidase 1 (Gpx1) and superoxide dismutase-1 (Sod1) by reverse-transcription polymerase chain reaction. Sperm motility was determined by Integrated Semen Analysis System (ISAS). Intermittent hypoxia significantly increased testicular oxidative stress, showing a reduction in the expression of Gpx1 and Sod1 by 38.9% and 34.4%, respectively, as compared with normoxia (P < 0.05). Progressive sperm motility was significantly reduced from 27.0 ± 6.4% in normoxia to 12.8 ± 1.8% in the intermittent hypoxia group (P = 0.04). The proportion of pregnant females and number of fetuses per mating was significantly lower in the intermittent hypoxia group (0.33 ± 0.10 and 2.45 ± 0.73, respectively) than in normoxic controls (0.72 ± 0.16 and 5.80 ± 1.24, respectively).

CONCLUSIONS:

These results suggest that the intermittent hypoxia associated with obstructive sleep apnea (OSA) could induce fertility reduction in male patients with this sleep breathing disorder.

KEYWORDS:

hypoxia; male fertility; obstructive sleep apnea; oxidative stress

PMID:
25364071
PMCID:
PMC4196059
DOI:
10.5665/sleep.4166
[Indexed for MEDLINE]
Free PMC Article

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