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Nat Neurosci. 2014 Dec;17(12):1673-81. doi: 10.1038/nn.3858. Epub 2014 Nov 2.

Glial ankyrins facilitate paranodal axoglial junction assembly.

Author information

1
Program in Developmental Biology, Baylor College of Medicine, Houston, Texas, USA.
2
Department of Neuroscience, Baylor College of Medicine, Houston, Texas, USA.
3
Centre for Neuroregeneration, University of Edinburgh, Edinburgh, UK.
4
Departments of Physiology and Cell Biology, Internal Medicine, and the Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio, USA.
5
1] Department of Neuroscience, Baylor College of Medicine, Houston, Texas, USA. [2] Department of Neurology, Baylor College of Medicine, Houston, Texas, USA.
6
Department of Cell Biology, Duke University, Durham, North Carolina, USA.
7
1] Program in Developmental Biology, Baylor College of Medicine, Houston, Texas, USA. [2] Department of Neuroscience, Baylor College of Medicine, Houston, Texas, USA.

Abstract

Neuron-glia interactions establish functional membrane domains along myelinated axons. These include nodes of Ranvier, paranodal axoglial junctions and juxtaparanodes. Paranodal junctions are the largest vertebrate junctional adhesion complex, and they are essential for rapid saltatory conduction and contribute to assembly and maintenance of nodes. However, the molecular mechanisms underlying paranodal junction assembly are poorly understood. Ankyrins are cytoskeletal scaffolds traditionally associated with Na(+) channel clustering in neurons and are important for membrane domain establishment and maintenance in many cell types. Here we show that ankyrin-B, expressed by Schwann cells, and ankyrin-G, expressed by oligodendrocytes, are highly enriched at the glial side of paranodal junctions where they interact with the essential glial junctional component neurofascin 155. Conditional knockout of ankyrins in oligodendrocytes disrupts paranodal junction assembly and delays nerve conduction during early development in mice. Thus, glial ankyrins function as major scaffolds that facilitate early and efficient paranodal junction assembly in the developing CNS.

PMID:
25362471
PMCID:
PMC4260775
DOI:
10.1038/nn.3858
[Indexed for MEDLINE]
Free PMC Article

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