Universal probe amplification: multiplex screening technologies for genetic variations

Biotechnol J. 2015 Jan;10(1):45-55. doi: 10.1002/biot.201400219. Epub 2014 Oct 28.

Abstract

In order to achieve multiplex screening of genetic variations, multiplex amplification of target genomic DNA is necessary. Universal amplification technology meets this requirement by simultaneously amplifying a number of different regions within the target genomic DNA using a single pair of universal primers and thus eliminating the limitations associated with the use of multiple pairs of primers. We comprehensively review universal probe amplification and its use with multiplex technologies for the identification of the most representative genetic variation, i. e. single nucleotide polymorphisms. The progress and key issues relating to universal probe amplification are discussed and the representative technologies are summarized with an emphasis on their application for the identification of susceptibility to human diseases.

Keywords: Analytical biotechnology; Genetic variation; Microarrays; Multiplex screening; Universal amplification.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Biotechnology
  • Electrophoresis, Capillary
  • Genetic Variation
  • Molecular Probe Techniques*
  • Molecular Probes*
  • Nucleic Acid Amplification Techniques / methods*
  • Oligonucleotide Array Sequence Analysis
  • Research Design

Substances

  • Molecular Probes