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Sci Rep. 2014 Oct 28;4:6796. doi: 10.1038/srep06796.

Mapping biological composition through quantitative phase and absorption X-ray ptychography.

Author information

1
ARC Centre of Excellence for Coherent X-Ray Science, Department of Physics, La Trobe University, Bundoora 3086, Australia.
2
ARC Centre of Excellence for Coherent X-Ray Science, Department of Biochemistry, La Trobe University, Bundoora 3086, Australia.
3
1] ARC Centre of Excellence for Coherent X-Ray Science, Department of Physics, La Trobe University, Bundoora 3086, Australia [2] Australian Synchrotron, 800 Blackburn Rd, Clayton 3168, Australia [3] ARC Centre of Excellence for Advanced Molecular Imaging, Australian Synchrotron, 800 Blackburn Rd, Clayton 3168, Australia.

Abstract

Isolating compositional information in biological X-ray imaging can be problematic as such information is conflated with thickness and density variations when viewing in projection through a sample. We demonstrate an effective method for identifying variations in material composition by simultaneously using the quantitative phase and magnitude images provided through soft X-ray ptychography. Using this approach we show significantly increased contrast and improved reliability of the identification of intracellular features from uncharacterised samples. While demonstrated for X-ray ptychography, this method is immediately applicable to electron and optical microscopy methods where the complex transmission function of the sample is recovered.

PMID:
25348877
PMCID:
PMC4210942
DOI:
10.1038/srep06796
[Indexed for MEDLINE]
Free PMC Article

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