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Pediatr Nephrol. 2015 Apr;30(4):677-85. doi: 10.1007/s00467-014-2989-y. Epub 2014 Oct 28.

Pediatric reference ranges for acute kidney injury biomarkers.

Author information

1
Biomarker Laboratory, Division of Nephrology and Hypertension, Cincinnati Children's Hospital Medical Center, ML 7022, 3333 Burnet Avenue, Cincinnati, OH, 45229, USA, michael.bennett@cchmc.org.

Abstract

BACKGROUND:

Novel urinary biomarkers are useful for the prediction of acute kidney injury (AKI). Most promising are the urine markers NGAL, IL-18, KIM-1, and LFABP. Each of these has shown considerable promise diagnosing AKI earlier than serum creatinine (Scr) using disease controls. We set out to determine reference levels of these markers in a healthy pediatric population.

METHODS:

Urine was collected from 368 healthy children and assayed for NGAL, IL-18, KIM-1, and LFABP using commercially available kits or assay materials. Analysis of biomarkers by linear regression and according to age groups (3-<5 years; 5-<10; 10-<15; 15-<18) was performed to determine if biomarker levels differed with age and gender.

RESULTS:

Median values were: NGAL (6.6 ng/ml; IQR 2.8-17), IL-18 (21.6 pg/ml; IQR 13.6-32.9), KIM-1 (410 pg/ml; IQR 226-703), LFABP (3.4 ng/ml; IQR 1.6-6.0). Significant gender differences were found with NGAL and IL-18 and significant age differences were found with all markers. 95th percentile values for each marker varied with age and gender greater than median values.

CONCLUSIONS:

This is the largest pediatric reference range study for the urinary measurement of NGAL, IL-18, KIM-1, and LFABP and highlights age and gender differences in these markers. This information is essential for rational interpretation of studies and clinical trials utilizing these emerging AKI biomarkers.

PMID:
25348707
PMCID:
PMC4504213
DOI:
10.1007/s00467-014-2989-y
[Indexed for MEDLINE]
Free PMC Article

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