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Angew Chem Int Ed Engl. 2014 Nov 17;53(47):12735-40. doi: 10.1002/anie.201405991. Epub 2014 Oct 24.

Facile and scalable preparation of pure and dense DNA origami solutions.

Author information

1
Physik Department, Walter Schottky Institute, Technische Universität München, Am Coulombwall 4a, 85748 Garching (Germany) http://bionano.physik.tu-muenchen.de.

Abstract

DNA has become a prime material for assembling complex three-dimensional objects that promise utility in various areas of application. However, achieving user-defined goals with DNA objects has been hampered by the difficulty to prepare them at arbitrary concentrations and in user-defined solution conditions. Here, we describe a method that solves this problem. The method is based on poly(ethylene glycol)-induced depletion of species with high molecular weight. We demonstrate that our method is applicable to a wide spectrum of DNA shapes and that it achieves excellent recovery yields of target objects up to 97 %, while providing efficient separation from non-integrated DNA strands. DNA objects may be prepared at concentrations up to the limit of solubility, including the possibility for bringing DNA objects into a solid phase. Due to the fidelity and simplicity of our method we anticipate that it will help to catalyze the development of new types of applications that use self-assembled DNA objects.

KEYWORDS:

DNA nanotechnology; DNA origami; molecular crowding; poly(ethylene glycol); self-assembly

PMID:
25346175
PMCID:
PMC4253120
DOI:
10.1002/anie.201405991
[Indexed for MEDLINE]
Free PMC Article

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