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Front Cell Neurosci. 2014 Oct 7;8:315. doi: 10.3389/fncel.2014.00315. eCollection 2014.

Methylene blue promotes quiescence of rat neural progenitor cells.

Author information

1
Department of Pharmacology and Neuroscience, University of North Texas Health Science Center Fort Worth, TX, USA.
2
Department of Neurosurgery, Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University Beijing, China.
3
Department of Molecular Biology, University of Texas Southwestern Medical Center Dallas, TX, USA.
4
Department of Pharmacology and Neuroscience, University of North Texas Health Science Center Fort Worth, TX, USA ; Department of Neurosurgery, Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University Beijing, China.

Abstract

Neural stem cell-based treatment holds a new therapeutic opportunity for neurodegenerative disorders. Here, we investigated the effect of methylene blue on proliferation and differentiation of rat neural progenitor cells (NPCs) both in vitro and in vivo. We found that methylene blue inhibited proliferation and promoted quiescence of NPCs in vitro without affecting committed neuronal differentiation. Consistently, intracerebroventricular infusion of methylene blue significantly inhibited NPC proliferation at the subventricular zone (SVZ). Methylene blue inhibited mTOR signaling along with down-regulation of cyclins in NPCs in vitro and in vivo. In summary, our study indicates that methylene blue may delay NPC senescence through enhancing NPCs quiescence.

KEYWORDS:

methylene blue; neural progenitor cell; neurogenesis; proliferation; quiescence

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