Format

Send to

Choose Destination
See comment in PubMed Commons below
J Cancer Prev. 2014 Mar;19(1):14-22.

Polyphenols Isolated from Allium cepa L. Induces Apoptosis by Induction of p53 and Suppression of Bcl-2 through Inhibiting PI3K/Akt Signaling Pathway in AGS Human Cancer Cells.

Author information

1
Departments of Internal Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju.
2
Emergency Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju.
3
Pharmacology, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju.
4
Department of Surgery, Konkuk University School of Medicine, Seoul.
5
School of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University.
6
Division of Applied Life Science (BK 21 Program), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju.
7
Department of Chemistry, Research Institute of Life Science, Gyeongsang National University.
8
Surgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju.
9
Department of Biochemistry, Dongeui University College of Oriental Medicine and Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University, Busan, Korea.
10
Neurosurgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju.

Abstract

BACKGROUND:

The extract of Allium cepa Linn is commonly used as adjuvant food for cancer therapy. We assumed that it includes a potential source of anti-cancer properties.

METHODS:

We investigated anti-cancer effects of polyphenols extracted from lyophilized A. cepa Linn (PEAL) in AGS human cancer cells.

RESULTS:

PEAL inhibited cell growth in a dose-dependent manner. It was related to caspase-dependent apoptosis. We confirmed this finding with annexin V staining. PEAL up-regulated p53 expression, and subsequent Bax induction, down regulated Bcl-2 protein, anti-apoptotic protein. In addition, PEAL suppressed Akt activity and PEAL-induced apoptosis were significantly accentuated with Akt inhibitor (LY294002).

CONCLUSIONS:

Our data suggested that PEAL induce caspase-dependent apoptosis through mitochondrial pathway by up-regulating p53 protein, and subsequent Bax protein as well as by modulating Bcl-2 protein, and that PEAL induces caspase-dependent apoptosis at least in part through the inhibition of phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway. This study provides evidence that PEAL might be useful for the treatment of cancer.

KEYWORDS:

Akt; Allium cepa; Apoptosis; Bcl-2; polyphenols

PMID:
25337568
PMCID:
PMC4189478
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for PubMed Central
    Loading ...
    Support Center