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Bioanalysis. 2014 Aug;6(16):2125-33. doi: 10.4155/bio.14.191.

Quantification of amikacin and kanamycin in serum using a simple and validated LC-MS/MS method.

Author information

1
University of Groningen, University Medical Center Groningen, Department of Clinical Pharmacy & Pharmacology, PO Box 30.001, 9700 RB Groningen, The Netherlands.

Abstract

BACKGROUND:

Amikacin and kanamycin are frequently used in the treatment of multidrug-resistant TB. The current commercially available immunoassay is unable to analyze kanamycin and trough levels of amikacin. The objective was therefore to develop a LC-MS/MS method for the quantification of amikacin and kanamycin in human serum.

MATERIALS & METHODS:

Using apramycin as internal standard, selectivity, accuracy, precision, recovery, matrix effects and stability were evaluated.

RESULTS:

The presented LC-MS/MS method meets the recommendations of the US FDA with a low LLOQ of 250 ng/ml for amikacin and 100 ng/ml for kanamycin. No statistical significant difference was found between the LC-MS/MS method and the immunoassay of amikacin (Architect(®) assay, p = 0.501).

CONCLUSION:

The low LLOQ of amikacin and the ability to analyze kanamycin makes the LC-MS/MS method the preferred method for analyzing these aminoglycosides.

PMID:
25331857
DOI:
10.4155/bio.14.191
[Indexed for MEDLINE]

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