Axonally derived matrilin-2 induces proinflammatory responses that exacerbate autoimmune neuroinflammation

J Clin Invest. 2014 Nov;124(11):5042-56. doi: 10.1172/JCI71385. Epub 2014 Oct 20.

Abstract

In patients with multiple sclerosis (MS) and mice with experimental autoimmune encephalomyelitis (EAE), inflammatory axonal injury is a major determinant of disability; however, the drivers of this injury are incompletely understood. Here, we used the EAE model and determined that the extracellular matrix protein matrilin-2 (MATN2) is an endogenous neuronal molecule that is regulated in association with inflammatory axonal injury. Compared with WT mice, mice harboring a deletion of Matn2 exhibited reduced disease severity and axon damage following induction of EAE. Evaluation of neuron-macrophage cocultures revealed that exogenous MATN2 specifically signals through TLR4 and directly induces expression of proinflammatory genes in macrophages, promoting axonal damage. Moreover, the MATN2-induced proinflammatory response was attenuated greatly in macrophages from Myd88 KO mice. Examination of brain sections from patients with MS revealed that MATN2 is expressed in lesions but not in normal-appearing white matter. Together, our results indicate that MATN2 is a deleterious endogenous neuroaxonal injury response signal that activates innate immune cells and could contribute to early axonal damage in CNS inflammatory diseases like MS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Axons / immunology
  • Axons / metabolism
  • Cells, Cultured
  • Coculture Techniques
  • Encephalomyelitis, Autoimmune, Experimental / metabolism*
  • Female
  • Humans
  • Immunity, Innate
  • Inflammation Mediators / metabolism
  • Macrophages / immunology
  • Macrophages / metabolism
  • Male
  • Matrilin Proteins / physiology
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Motor Cortex / metabolism
  • Signal Transduction
  • Spinal Cord Injuries / metabolism
  • Toll-Like Receptor 4 / metabolism
  • Transcriptional Activation

Substances

  • Inflammation Mediators
  • Matn2 protein, mouse
  • Matrilin Proteins
  • Tlr4 protein, mouse
  • Toll-Like Receptor 4