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Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1842-3. doi: 10.3109/19401736.2014.971251. Epub 2014 Oct 20.

Complete genome sequence of the mitochondrial DNA of the sparkling enope squid, Watasenia scintillans.

Author information

1
a Department of Maternal-Fetal Biology , National Research Institute for Child Health and Development , Tokyo , Japan .
2
b Division of Life Sciences , Graduate School of Humanities and Sciences, Ochanomizu University , Tokyo , Japan .
3
c Center for Informational Biology, Ochanomizu University , Tokyo , Japan .
4
d National Institute of Genetics , Mishima , Japan .
5
e Japan Society for the Promotion of Science , Tokyo , Japan .
6
f Nagahama Institute of Bio-Science and Technology , Nagahama , Japan , and.
7
g Department of Systems BioMedicine , National Research Institute for Child Health and Development , Tokyo , Japan.

Abstract

The sparkling enope squid, Watasenia scintillans, is a deep-sea mollusk inhabiting the western part of the Pacific Ocean. It has the peculiar ability to illuminate its body without the involvement of other organisms. In this study, we extracted the brain DNA from a single squid female caught in the Japan Sea and determined the complete genome sequence of its mitochondrial DNA using the Illumina sequencing platform. The circular sequence is 20,089 bp in length. Using the next-generation sequencing data, we also estimated the mean copy number of mitochondria per cell in the brain to be 108 by comparing the depths of the read data in the nuclear and mitochondrial genomes. The haploid genome size was calculated to be 4.78 Gb. Six heteroplasmy sites were also identified, together with their allele frequencies, in this individual. Our methodology is shown to be useful in mitochondrion-related studies.

KEYWORDS:

Cephalopod; Watasenia scintillans; genome size; mollusk; sparkling enope squid

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