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Methods Mol Biol. 2015;1229:549-55. doi: 10.1007/978-1-4939-1714-3_43.

Heparan sulfate modulates Slit3-induced endothelial cell migration.

Author information

1
Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA, 30602-4712, USA.

Abstract

Heparan sulfate is a long, linear polysaccharide with sulfation modifications and belongs to the glycosaminoglycan family. Our recent studies elucidated that the axon guidance molecule Slit3 is a new heparan sulfate-binding protein and a novel angiogenic factor by interacting with its cognate receptor Robo4, which is specifically expressed in endothelial cells. Here we describe using heparan sulfate-deficient mouse endothelial cells to determine the co-reception function of heparan sulfate in Slit3-induced endothelial cell migration in a Boyden chamber trans-well migration assay.

PMID:
25325980
DOI:
10.1007/978-1-4939-1714-3_43
[Indexed for MEDLINE]

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