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Biomacromolecules. 2014 Dec 8;15(12):4621-6. doi: 10.1021/bm501467h. Epub 2014 Nov 20.

Efficient targeting of fatty-acid modified oligonucleotides to live cell membranes through stepwise assembly.

Author information

1
Department of Pharmaceutical Chemistry, University of California, San Francisco , 600 16th Street, Box 2280, San Francisco, California 94158, United States.

Abstract

Lipid modifications provide efficient targeting of oligonucleotides to live cell membranes in a range of applications. Targeting efficiency is a function of the rate of lipid DNA insertion into the cell surface and its persistence over time. Here we show that increasing lipid hydrophobicity increases membrane persistence, but decreases the rate of membrane insertion due to the formation of nonproductive aggregates in solution. To ameliorate this effect, we split the net hydrophobicity of the membrane anchor between two complementary oligonucleotides. When prehybridized in solution, doubly anchored molecules also aggregate due to their elevated hydrophobicity. However, when added sequentially to cells, aggregation does not occur so membrane insertion is efficient. Hybridization between the two strands locks the complexes at the cell surface by increasing net hydrophobicity, increasing their total concentration and lifetime, and dramatically improving their utility in a variety of biomedical applications.

PMID:
25325667
PMCID:
PMC4261982
DOI:
10.1021/bm501467h
[Indexed for MEDLINE]
Free PMC Article

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