Format

Send to

Choose Destination
Front Plant Sci. 2014 Sep 30;5:484. doi: 10.3389/fpls.2014.00484. eCollection 2014.

Genotyping-by-sequencing (GBS), an ultimate marker-assisted selection (MAS) tool to accelerate plant breeding.

Author information

1
Inner Mongolia Academy of Agriculture and Husbandry Science Hohhot, China ; Lethbridge Research Centre, Agriculture and Agri-Food Canada Lethbridge, AB, Canada.
2
Inner Mongolia Academy of Agriculture and Husbandry Science Hohhot, China.
3
Lethbridge Research Centre, Agriculture and Agri-Food Canada Lethbridge, AB, Canada.

Abstract

Marker-assisted selection (MAS) refers to the use of molecular markers to assist phenotypic selections in crop improvement. Several types of molecular markers, such as single nucleotide polymorphism (SNP), have been identified and effectively used in plant breeding. The application of next-generation sequencing (NGS) technologies has led to remarkable advances in whole genome sequencing, which provides ultra-throughput sequences to revolutionize plant genotyping and breeding. To further broaden NGS usages to large crop genomes such as maize and wheat, genotyping-by-sequencing (GBS) has been developed and applied in sequencing multiplexed samples that combine molecular marker discovery and genotyping. GBS is a novel application of NGS protocols for discovering and genotyping SNPs in crop genomes and populations. The GBS approach includes the digestion of genomic DNA with restriction enzymes followed by the ligation of barcode adapter, PCR amplification and sequencing of the amplified DNA pool on a single lane of flow cells. Bioinformatic pipelines are needed to analyze and interpret GBS datasets. As an ultimate MAS tool and a cost-effective technique, GBS has been successfully used in implementing genome-wide association study (GWAS), genomic diversity study, genetic linkage analysis, molecular marker discovery and genomic selection under a large scale of plant breeding programs.

KEYWORDS:

genomic selection (GS); genotyping-by-sequencing (GBS); marker-assisted selection (MAS); next-generation sequencing (NGS); single nucleotide polymorphism (SNP)

Supplemental Content

Full text links

Icon for Frontiers Media SA Icon for PubMed Central
Loading ...
Support Center