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Acupunct Med. 2015 Feb;33(1):65-71. doi: 10.1136/acupmed-2014-010634. Epub 2014 Oct 16.

Contributions of nitric oxide and prostaglandins to the local increase in muscle blood flow following manual acupuncture in rats.

Author information

1
Department of Basic Acupuncture and Moxibustion, Meiji University of Integrative Medicine, Nantan-shi, Kyoto, Japan.
2
Department of Anatomy and Neuroscience, Hyogo College of Medicine, Nishinomiya-shi, Hyogo, Japan.
3
Department of Health Promoting and Geriatric Acupuncture and Moxibustion, Meiji University of Integrative Medicine, Nantan-shi, Kyoto, Japan.

Abstract

OBJECTIVE:

To investigate the contributions of nitric oxide (NO) and prostaglandins (PGs) to the increase in local muscle blood flow (MBF) observed following manual acupuncture (MA).

METHODS:

Male Sprague-Dawley rats (n=112; 250-310 g) were injected intraperitoneally with a non-selective NO synthase inhibitor (NG-nitro-L-arginine methyl ester hydrochloride: L-NAME; 10, 50 or 500 mg/kg), a non-selective cyclo-oxygenase inhibitor (indomethacin; 10, 50 or 500 mg/kg), a combination of L-NAME and indomethacin (500 mg/kg each) or saline only under urethane anaesthesia (1.2 g/kg). We used the sparrow pecking technique for 1 min with a stainless steel acupuncture needle (0.20×30 mm) as the acupuncture stimulation method. The stimulus point was on the right tibialis anterior muscle. (51)Chromium-labelled microspheres were used for MBF measurement.

RESULTS:

MA increased MBF in the saline-injected group (p<0.001). This increase was partially inhibited by L-NAME in a dose-dependent manner (p>0.05, p<0.05 and p<0.001 for 10, 50 and 500 mg/kg, respectively). On the other hand, indomethacin did not suppress the increase (p>0.05 each for 10, 50 and 500 mg/kg). No significant difference was observed between the inhibitory effects of combined administration of L-NAME and indomethacin and single administration of L-NAME (p>0.05).

CONCLUSIONS:

These results suggest that NO is a major factor in the MA-induced increase in MBF, while PGs do not contribute significantly to this increase. As complete inhibition was not achieved by administration of L-NAME±indomethacin, it appears that non-NO and non-PG vasodilators are additionally involved.

KEYWORDS:

ACUPUNCTURE

PMID:
25324276
DOI:
10.1136/acupmed-2014-010634
[Indexed for MEDLINE]
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