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Sci Rep. 2014 Oct 17;4:6664. doi: 10.1038/srep06664.

Development of an immunologically tolerated combination of fluorescent proteins for in vivo two-photon imaging.

Author information

1
National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.

Abstract

Combinations of fluorescent proteins (FPs) are routinely used for multi-parameter in vivo imaging experiments to visualize tagged proteins or cell populations of interest. Studies involving FPs are often limited by spectral overlap, toxicity, relative quantum efficiency, and the potential for immunological rejection upon transfer into a non-tolerant recipient. Here we evaluate the immunologic visibility of several commonly used FPs by the murine immune system and identify a spectrally compatible, immunologically tolerated combination of FPs well suited for in vivo two-photon imaging.

PMID:
25322934
PMCID:
PMC4200419
DOI:
10.1038/srep06664
[Indexed for MEDLINE]
Free PMC Article

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