Abstract
Immunoblotting for phosphorylated forms of receptor tyrosine kinases (RTKs) has been the mainstay of investigations on RTK signaling for the past two decades. Despite the development of quantitative mass spectrometry, reverse-phase protein array, and multiplex technologies, immunoblotting with phospho-specific antibodies is still used in parallel with these technologies and remains a powerful, and reproducible, method for interrogating signaling networks involving RTKs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Phospho-Specific / chemistry*
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Cell Line
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Electrophoresis, Polyacrylamide Gel
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ErbB Receptors / antagonists & inhibitors
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ErbB Receptors / genetics
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ErbB Receptors / metabolism
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Gene Expression Regulation*
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Humans
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Immunoblotting / methods*
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Phosphoproteins / antagonists & inhibitors
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Phosphoproteins / genetics*
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Phosphoproteins / metabolism
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Phosphorylation
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Protein Kinase Inhibitors / pharmacology
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Proto-Oncogene Proteins c-met / antagonists & inhibitors
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Proto-Oncogene Proteins c-met / genetics
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Proto-Oncogene Proteins c-met / metabolism
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Receptor, ErbB-2 / antagonists & inhibitors
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Receptor, ErbB-2 / genetics
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Receptor, ErbB-2 / metabolism
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Receptor, ErbB-3 / antagonists & inhibitors
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Receptor, ErbB-3 / genetics
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Receptor, ErbB-3 / metabolism
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Receptor, IGF Type 1
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Receptors, Somatomedin / antagonists & inhibitors
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Receptors, Somatomedin / genetics
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Receptors, Somatomedin / metabolism
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Signal Transduction
Substances
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Antibodies, Phospho-Specific
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IGF1R protein, human
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Phosphoproteins
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Protein Kinase Inhibitors
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Receptors, Somatomedin
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EGFR protein, human
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ERBB2 protein, human
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ERBB3 protein, human
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ErbB Receptors
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Proto-Oncogene Proteins c-met
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Receptor, ErbB-2
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Receptor, ErbB-3
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Receptor, IGF Type 1