Format

Send to

Choose Destination
Elife. 2014 Oct 14;3. doi: 10.7554/eLife.03558.

Mitochondrial fusion but not fission regulates larval growth and synaptic development through steroid hormone production.

Author information

1
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.
2
Program in Developmental Biology, Baylor College of Medicine, Houston, United States.
3
Howard Hughes Medical Institute, Baylor College of Medicine, Houston, United States.
4
Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, United States.

Abstract

Mitochondrial fusion and fission affect the distribution and quality control of mitochondria. We show that Marf (Mitochondrial associated regulatory factor), is required for mitochondrial fusion and transport in long axons. Moreover, loss of Marf leads to a severe depletion of mitochondria in neuromuscular junctions (NMJs). Marf mutants also fail to maintain proper synaptic transmission at NMJs upon repetitive stimulation, similar to Drp1 fission mutants. However, unlike Drp1, loss of Marf leads to NMJ morphology defects and extended larval lifespan. Marf is required to form contacts between the endoplasmic reticulum and/or lipid droplets (LDs) and for proper storage of cholesterol and ecdysone synthesis in ring glands. Interestingly, human Mitofusin-2 rescues the loss of LD but both Mitofusin-1 and Mitofusin-2 are required for steroid-hormone synthesis. Our data show that Marf and Mitofusins share an evolutionarily conserved role in mitochondrial transport, cholesterol ester storage and steroid-hormone synthesis.

KEYWORDS:

Charcot-Marie-Tooth type 2A; Drp1; Mfn1 and Mfn2; Opa1; developmental biology; drosophila melanogaster; endoplasmic reticulum; lipid droplets; mitochondria transport; neuroscience; stem cells

PMID:
25313867
PMCID:
PMC4215535
DOI:
10.7554/eLife.03558
[Indexed for MEDLINE]
Free PMC Article

Publication types, MeSH terms, Substances, Grant support

Publication types

MeSH terms

Substances

Grant support

Supplemental Content

Full text links

Icon for eLife Sciences Publications, Ltd Icon for PubMed Central
Loading ...
Support Center