Format

Send to

Choose Destination
J Cell Biol. 2014 Oct 13;207(1):107-21. doi: 10.1083/jcb.201402037.

Isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells.

Author information

1
Cellular Dynamics and Cell Patterning and Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany Institute of Cell Dynamics and Imaging and Cells-In-Motion Cluster of Excellence (EXC1003-CiM), University of Münster, 48149 Münster, Germany Institute of Cell Dynamics and Imaging and Cells-In-Motion Cluster of Excellence (EXC1003-CiM), University of Münster, 48149 Münster, Germany.
2
Cellular Dynamics and Cell Patterning and Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany.
3
Institute of Cell Dynamics and Imaging and Cells-In-Motion Cluster of Excellence (EXC1003-CiM), University of Münster, 48149 Münster, Germany Institute of Cell Dynamics and Imaging and Cells-In-Motion Cluster of Excellence (EXC1003-CiM), University of Münster, 48149 Münster, Germany.
4
Laboratory for Computational Biology & Biophysics, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139.
5
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
6
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany Division of Molecular Biology, Ruđer Bošković Institute, 10000 Zagreb, Croatia.
7
Cellular Dynamics and Cell Patterning and Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany Institute of Cell Dynamics and Imaging and Cells-In-Motion Cluster of Excellence (EXC1003-CiM), University of Münster, 48149 Münster, Germany Institute of Cell Dynamics and Imaging and Cells-In-Motion Cluster of Excellence (EXC1003-CiM), University of Münster, 48149 Münster, Germany wedlich@uni-muenster.de.

Abstract

Although cortical actin plays an important role in cellular mechanics and morphogenesis, there is surprisingly little information on cortex organization at the apical surface of cells. In this paper, we characterize organization and dynamics of microvilli (MV) and a previously unappreciated actomyosin network at the apical surface of Madin-Darby canine kidney cells. In contrast to short and static MV in confluent cells, the apical surfaces of nonconfluent epithelial cells (ECs) form highly dynamic protrusions, which are often oriented along the plane of the membrane. These dynamic MV exhibit complex and spatially correlated reorganization, which is dependent on myosin II activity. Surprisingly, myosin II is organized into an extensive network of filaments spanning the entire apical membrane in nonconfluent ECs. Dynamic MV, myosin filaments, and their associated actin filaments form an interconnected, prestressed network. Interestingly, this network regulates lateral mobility of apical membrane probes such as integrins or epidermal growth factor receptors, suggesting that coordinated actomyosin dynamics contributes to apical cell membrane organization.

Comment in

PMID:
25313407
PMCID:
PMC4195824
DOI:
10.1083/jcb.201402037
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center