Abstract
The four major surface antigens of Toxoplasma gondii tachyzoites (P43, P35, P30, and P22) were made water soluble by phosphatidylinositol-specific phospholipase C (PI-PLC). These antigens were biosynthetically labeled with 3H-fatty acids, [3H]ethanolamine, and [3H]carbohydrates. Treatment of 3H-fatty-acid-labeled parasite lysates with PI-PLC removed the radioactive label from these antigens. A cross-reacting determinant was exposed on these antigens after PI-PLC treatment.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antigens, Protozoan / analysis*
-
Antigens, Surface / analysis*
-
Electrophoresis, Polyacrylamide Gel
-
Ethanolamines / metabolism
-
Glucosamine / metabolism
-
Glycolipids / analysis*
-
Glycosylphosphatidylinositols
-
Iodine Radioisotopes
-
Isotope Labeling
-
Mannose / metabolism
-
Mice
-
Myristic Acids / metabolism
-
Palmitic Acids / metabolism
-
Phosphatidylinositols / analysis*
-
Precipitin Tests
-
Toxoplasma / immunology*
-
Type C Phospholipases
Substances
-
Antigens, Protozoan
-
Antigens, Surface
-
Ethanolamines
-
Glycolipids
-
Glycosylphosphatidylinositols
-
Iodine Radioisotopes
-
Myristic Acids
-
Palmitic Acids
-
Phosphatidylinositols
-
Type C Phospholipases
-
Glucosamine
-
Mannose