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Appl Plant Sci. 2014 Oct 2;2(10). pii: apps.1400049. doi: 10.3732/apps.1400049. eCollection 2014 Oct.

Primers for low-copy nuclear genes in Metrosideros and cross-amplification in Myrtaceae.

Author information

1
Tropical Conservation Biology and Environmental Science Program, University of Hawai'i at Hilo, 200 West Kawili Street, Hilo, Hawai'i 96720 USA.
2
Department of Biology, University of Florida, Gainesville, Florida 32611 USA ; Genetics Institute, University of Florida, Gainesville, Florida 32610 USA.

Abstract

PREMISE OF THE STUDY:

Primers were developed to amplify low-copy nuclear genes in Hawaiian Metrosideros (Myrtaceae). •

METHODS AND RESULTS:

Data from a pooled 454 Titanium run of the partial transcriptomes of four Metrosideros taxa were used to identify the loci of interest. Ten exon-primed intron-crossing (EPIC) markers were amplified and sequenced directly with success in Metrosideros, as well as in a representative selection of Myrtaceae, including Syzygium, Psidium, and Melaleuca for most of the markers. The loci amplified ranged between 500 and 1100 bp, and up to 117 polymorphic sites were observed within an individual gene alignment. Two introns contained microsatellites in some of the species. •

CONCLUSIONS:

These novel primer pairs should be useful for phylogenetic analysis and population genetics of a broad range of Myrtaceae, particularly the diverse fleshy-fruited tribes Syzygieae and Myrteae.

KEYWORDS:

Hawai‘i; Metrosideros; Myrtaceae; next-generation sequencing; phylogeny; single nuclear genes

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