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Methods Mol Biol. 2015;1241:23-38. doi: 10.1007/978-1-4939-1875-1_3.

PCR based determination of mitochondrial DNA copy number in multiple species.

Author information

1
Nicholas School of the Environment, Duke University, 450 Research Dr., Durham, NC, 27708-0328, USA.

Abstract

Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA (nucDNA) and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material and availability of specific PCR reagents.

PMID:
25308485
PMCID:
PMC4312664
DOI:
10.1007/978-1-4939-1875-1_3
[Indexed for MEDLINE]
Free PMC Article

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