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Chem Biol. 2014 Nov 20;21(11):1557-63. doi: 10.1016/j.chembiol.2014.08.018. Epub 2014 Oct 9.

Structural and functional analysis of Bacillus subtilis YisP reveals a role of its product in biofilm production.

Author information

1
Department of Chemistry, University of Illinois, Urbana, IL 61801, USA.
2
Industrial Enzymes National Engineering Lab, Tianjin Institute of Industrial Biotechnology, Tianjin 300308, China.
3
Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan.
4
Interfaculty Institute for Microbiology and Infection Medicine Tübingen, University of Tübingen, Tübingen 72076, Germany.
5
Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115, USA.
6
Industrial Enzymes National Engineering Lab, Tianjin Institute of Industrial Biotechnology, Tianjin 300308, China. Electronic address: guo_rt@tib.cas.cn.
7
Department of Chemistry, University of Illinois, Urbana, IL 61801, USA. Electronic address: eo@chad.scs.uiuc.edu.

Abstract

YisP is involved in biofilm formation in Bacillus subtilis and has been predicted to produce C30 isoprenoids. We determined the structure of YisP and observed that it adopts the same fold as squalene and dehydrosqualene synthases. However, the first aspartate-rich motif found in essentially all isoprenoid synthases is aspartate poor in YisP and cannot catalyze head-to-head condensation reactions. We find that YisP acts as a phosphatase, catalyzing formation of farnesol from farnesyl diphosphate, and that it is the first phosphatase to adopt the fold seen in the head-to-head prenyl synthases. Farnesol restores biofilm formation in a Δyisp mutant and modifies lipid membrane structure similarly to the virulence factor staphyloxanthin. This work clarifies the role of YisP in biofilm formation and suggests an intriguing possibility that many of the YisP-like homologs found in other bacteria may also have interesting products and functions.

Comment in

PMID:
25308276
PMCID:
PMC4252625
DOI:
10.1016/j.chembiol.2014.08.018
[Indexed for MEDLINE]
Free PMC Article

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