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Braz J Infect Dis. 2014 Nov-Dec;18(6):672-4. doi: 10.1016/j.bjid.2014.08.004. Epub 2014 Oct 11.

A search for Clostridium difficile ribotypes 027 and 078 in Brazil.

Author information

1
PPG Patologia, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.
2
PPG Patologia, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil; Santa Casa de Misericórdia de Porto Alegre, Porto Alegre, RS, Brazil.
3
Statens Serum Institut, Copenhagen, Denmark.
4
Grupo Hospitalar Conceição, Porto Alegre, RS, Brazil.
5
PPG Patologia, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil; Santa Casa de Misericórdia de Porto Alegre, Porto Alegre, RS, Brazil. Electronic address: acpasqualotto@hotmail.com.

Abstract

Toxigenic strains of Clostridium difficile may be disseminating. Here we prospectively screened patients with nosocomial diarrhoea in two hospitals in Brazil. To identify C. difficile polymerase chain reaction ribotypes 027/078 strains, we used high resolution melting and multiplex polymerase chain reaction. Among 116 screened patients, 11 were positive for C. difficile. The polymerase chain reaction ribotypes 027/078 strains were not identified in this study.

KEYWORDS:

Clostridium difficile; HRM; Multiplex-PCR; Toxin gene profiling

PMID:
25307680
DOI:
10.1016/j.bjid.2014.08.004
[Indexed for MEDLINE]
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