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Dev Cell. 2014 Oct 27;31(2):240-7. doi: 10.1016/j.devcel.2014.08.015. Epub 2014 Oct 9.

The Dpp/TGFβ-dependent corepressor Schnurri protects epithelial cells from JNK-induced apoptosis in drosophila embryos.

Author information

1
Medical Research Council National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK; Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK. Electronic address: jbeira@gmail.com.
2
BIOSS Centre for Biological Signalling Studies and Institute for Biology I, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany; Spemann Graduate School of Biology and Medicine, Research Training Program GRK 1104, Albert Ludwigs University, 79104 Freiburg, Germany.
3
European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
4
BIOSS Centre for Biological Signalling Studies and Institute for Biology I, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany.
5
Medical Research Council National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK. Electronic address: jvincen@nimr.mrc.ac.uk.

Abstract

Jun N-terminal kinase (JNK) often mediates apoptosis in response to cellular stress. However, during normal development, JNK signaling controls a variety of live cell behaviors, such as during dorsal closure in Drosophila embryos. During this process, the latent proapoptotic activity of JNK becomes apparent following Dpp signaling suppression, which leads to JNK-dependent transcriptional activation of the proapoptotic gene reaper. Dpp signaling also protects cells from JNK-dependent apoptosis caused by epithelial disruption. We find that repression of reaper transcription by Dpp is mediated by Schnurri. Moreover, reporter gene analysis shows that a transcriptional regulatory module comprising AP-1 and Schnurri binding sites located upstream of reaper integrate the activities of JNK and Dpp. This arrangement allows JNK to control a migratory behavior without triggering apoptosis. Dpp plays a dual role during dorsal closure. It cooperates with JNK in stimulating cell migration and also prevents JNK from inducing apoptosis.

PMID:
25307481
PMCID:
PMC4220000
DOI:
10.1016/j.devcel.2014.08.015
[Indexed for MEDLINE]
Free PMC Article

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