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Nat Methods. 2014 Dec;11(12):1233-6. doi: 10.1038/nmeth.3143. Epub 2014 Oct 12.

An improved surface passivation method for single-molecule studies.

Author information

1
Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
2
1] Department of Physics, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. [2] Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. [3] Howard Hughes Medical Institute, Urbana, Illinois, USA.
3
1] Department of Physics, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. [2] Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
4
1] School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea. [2] Center for Soft and Living Matter, Institute for Basic Science, Ulsan, Republic of Korea.
5
1] Howard Hughes Medical Institute, Urbana, Illinois, USA. [2] Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
6
T.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland, USA.
7
1] T.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland, USA. [2] Program in Cell, Molecular, Developmental Biology, and Biophysics (CMDB), Johns Hopkins University, Baltimore, Maryland, USA.
8
1] Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. [2] Department of Physics, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. [3] Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. [4] Howard Hughes Medical Institute, Urbana, Illinois, USA. [5] Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.

Abstract

We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly(ethylene glycol) surface. The DDS-Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution.

PMID:
25306544
PMCID:
PMC4245390
DOI:
10.1038/nmeth.3143
[Indexed for MEDLINE]
Free PMC Article

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