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Life Sci. 2014 Nov 4;117(1):13-8. doi: 10.1016/j.lfs.2014.09.005. Epub 2014 Oct 8.

Milk thistle impedes the development of carbontetrachloride-induced liver damage in rats through suppression of bcl-2 and regulating caspase pathway.

Author information

1
Firat University, Faculty of Science, Department of Biology, Elazığ, Turkey. Electronic address: aaslan@firat.edu.tr.
2
Aksaray University, Department of Biotechnology and Molecular Biology, Aksaray, Turkey. Electronic address: is_mail_can@hotmail.com.

Abstract

AIM:

The objective of this study was to examine whether MT plays a protective role against the damage in the liver by administering carbontetrachloride (CCl4) to rats.

MAIN METHOD:

28 male Wistar albino (n=28, 8weeks old) rats have been used in the study. The rats were distributed into 4 groups according to their live weights. The groups were: (i) negative control (NC): normal water consuming group to which no CCl4 and milk thistle (MT) is administered; (ii) positive control (PC): normal water consuming group to which no CCl4 is administered but MT is administered; (iii) CCl4 group: normal water consuming and group to which CCl4 is administered (2ml/kg live weight, ip); and (iv) CCl4+MT group: CCl4 and MT administered group (2ml/kg live weight, ip). Caspase-3, caspase-9, bax, and bcl-2 protein syntheses were examined via western blotting. MDA determination in liver tissue was made using spectrophotometer.

KEY FINDINGS:

MDA amount has decreased in the CCl4+MT group in comparison to CCl4 group whereas caspase-3 and caspase-9 has increased and bax and bcl-2 has decreased.

SIGNIFICANCE:

These results show that MT protects the liver against oxidative damage.

KEYWORDS:

Bax; Bcl-2; Caspase-3; Caspase-9; Liver damage; Milk thistle

PMID:
25305509
DOI:
10.1016/j.lfs.2014.09.005
[Indexed for MEDLINE]

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