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Brain Res. 2014 Dec 10;1592:22-33. doi: 10.1016/j.brainres.2014.09.072. Epub 2014 Oct 7.

Impact of microRNA-134 on neural cell survival against ischemic injury in primary cultured neuronal cells and mouse brain with ischemic stroke by targeting HSPA12B.

Author information

1
Department of Anesthesiology, Weifang Medical University, Weifang City 261053, Shangdong Province, PR China.
2
Department of Anesthesiology, Shandong University Affiliated Jinan City Central Hospital, Jinan 250013, PR China. Electronic address: mfj15318816233@163.com.
3
Department of Anesthesiology, Shandong University Affiliated Jinan City Central Hospital, Jinan 250013, PR China.
4
Department of Burn Surgery, Shandong University Affiliated Jinan City Central Hospital, Jinan 250013, PR China.
5
Department of Neurobiology and Beijing Institute for Brain Disorders, Capital Medical University, Beijing 100069, PR China.
6
Department of Neurobiology and Beijing Institute for Brain Disorders, Capital Medical University, Beijing 100069, PR China. Electronic address: junfali@ccmu.edu.cn.

Abstract

As a newly discovered member of the HSP70 family, heat shock protein A12B (HSPA12B) is involved in brain ischemic injury. According to our previous study, microRNA-134 (miR-134) could target HSPA12B by binding to its 3'-untranslated region (UTR). However, the regulation of miR-134 on HSPA12B and their role in protecting neuronal cells from ischemic injury are unclear. In this study, the miR-134 expression level was manipulated, and the HSPA12B protein levels were also determined in oxygen-glucose deprivation (OGD)-treated primary cultured neuronal cells in vitro and mouse brain after middle cerebral artery occlusion (MCAO)-induced ischemic stroke in vivo. The results showed that miR-134 expression levels increased in primary cultured neuronal cells and mouse brain from 12h to 7 day reoxygenation/reperfusion after 1h OGD or 1h MCAO treatment. miR-134 overexpression promoted neuronal cell death and apoptosis by decreasing HSPA12B protein levels. Conversely, downregulating miR-134 reduced neuronal cell death and apoptosis by enhancing HSPA12B protein levels. Also, HSPA12B siRNA could block miR-134 inhibitor-mediated neuroprotection against OGD-induced neuronal cell injury in vitro. Taken together, miR-134 might influence neuronal cell survival against ischemic injury in primary cultured neuronal cells and mouse brain with ischemic stroke by negatively modulating HSPA12B protein expression in a posttranscriptional manner.

KEYWORDS:

Heat shock protein A12B (HSPA12B); Ischemic stroke; MicroRNA-134 (miR-134); Middle cerebral artery occlusion (MCAO); Oxygen-glucose deprivation (OGD)

PMID:
25304362
DOI:
10.1016/j.brainres.2014.09.072
[Indexed for MEDLINE]

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