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Food Chem Toxicol. 2014 Dec;74:131-8. doi: 10.1016/j.fct.2014.09.002. Epub 2014 Oct 7.

Differential protective effects of extra virgin olive oil and corn oil in liver injury: a proteomic study.

Author information

1
School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei, China; Food and Nutrition Division, School of Biological Sciences, The University of Hong Kong, Hong Kong, China.
2
Food and Nutrition Division, School of Biological Sciences, The University of Hong Kong, Hong Kong, China.
3
Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
4
Food and Nutrition Division, School of Biological Sciences, The University of Hong Kong, Hong Kong, China. Electronic address: jmfwan@hku.hk.

Abstract

Extra virgin olive oil (EVOO) presents benefits against chronic liver injury induced by hepatotoxins such as carbon tetrachloride (CCl4); however, the protective mechanisms remain unclear. In the present study, a two-dimensional gel based proteomic approach was constructed to explore the mechanisms. Rats are injected with CCl4 twice a week for 4 weeks to induce liver fibrosis, and were fed laboratory chow plus 20% (w/w) of either corn oil or EVOO over the entire experimental period. Histological staining, MDA assay and fibrogenesis marker gene analysis illustrate that the CCl4-treated animals fed EVOO have a lower fibrosis and lipid peroxidation level in the liver than the corn oil fed group. The proteomic study indicates that the protein expression of thioredoxin domain-containing protein 12, peroxiredoxin-1, thiosulphate sulphurtransferase, calcium-binding protein 1, Annexin A2 and heat shock cognate 71‚ÄČkDa protein are higher in livers from EVOO-fed rats with the CCl4 treatment compared with those from rats fed with corn oil, whereas the expression of COQ9, cAMP-dependent protein kinase type I-alpha regulatory subunit, phenylalanine hydroxylase and glycerate kinase are lower. Our findings confirmed the benefits of EVOO against chronic liver injury, which may be attributable to the antioxidant effects, hepatocellular function regulation and hepatic metabolism modification effects of EVOO.

KEYWORDS:

Carbon tetrachloride; Corn oil; Extra virgin olive oil; Liver; Proteomics

PMID:
25303780
DOI:
10.1016/j.fct.2014.09.002
[Indexed for MEDLINE]

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