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J Cell Sci. 2014 Dec 1;127(Pt 23):5007-13. doi: 10.1242/jcs.136457. Epub 2014 Oct 9.

MAP1S controls microtubule stability throughout the cell cycle in human cells.

Author information

1
Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany.
2
European Molecular Biology Laboratory (EMBL), Advanced Light Microscopy Facility Programme, Meyerhostr.1, 69117 Heidelberg, Germany.
3
European Molecular Biology Laboratory (EMBL), Cell Biology and Biophysics Programme, Meyerhostr.1, 69117 Heidelberg, Germany.
4
Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany o.gruss@zmbh.uni-heidelberg.de.

Abstract

Understanding the molecular basis for proper cell division requires a detailed functional analysis of microtubule (MT)-associated proteins. MT-associated protein 1S (MAP1S), the most ubiquitously expressed MAP1 family member, is required for accurate cell division. Here, using quantitative analysis of MT plus-end tracking, we show that MAP1S knockdown alters MT dynamics throughout the cell cycle. Surprisingly, MAP1S downregulation results in faster growing, yet short-lived, MTs in all cell cycle stages and in a global loss of MT acetylation. These aberrations correlate with severe defects in the final stages of cell division. In monopolar cytokinesis assays, we demonstrate that MAP1S guides MT-dependent initiation of cytokinesis. Our data underline the key role of MAP1S as a global regulator of MT stability and demonstrate a new primary function of MAP1S to regulate MT dynamics at the onset of cytokinesis.

KEYWORDS:

Cytokinesis; MAP1S; Microtubule; Microtubule dynamics; Microtubule-associated protein

PMID:
25300793
DOI:
10.1242/jcs.136457
[Indexed for MEDLINE]
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