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PLoS One. 2014 Oct 9;9(10):e109976. doi: 10.1371/journal.pone.0109976. eCollection 2014.

NK cell phenotypic modulation in lung cancer environment.

Author information

1
Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Micro-environment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, P.R. China; Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin, P.R. China.
2
Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Micro-environment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, P.R. China; Cancer Center, Research Institute of Surgery and Daping Hospital, Third Military Medical University, Chongqing, P.R. China.
3
Department of neurology, Tianjin Medical University General Hospital, Tianjin, P.R. China.
4
Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Micro-environment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, P.R. China.
5
Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin, P.R. China.

Abstract

BACKGROUND:

Nature killer (NK) cells play an important role in anti-tumor immunotherapy. But it indicated that tumor cells impacted possibly on NK cell normal functions through some molecules mechanisms in tumor microenvironment.

MATERIALS AND METHODS:

Our study analyzed the change about NK cells surface markers (NK cells receptors) through immunofluorescence, flow cytometry and real-time PCR, the killed function from mouse spleen NK cell and human high/low lung cancer cell line by co-culture. Furthermore we certificated the above result on the lung cancer model of SCID mouse.

RESULTS:

We showed that the infiltration of NK cells in tumor periphery was related with lung cancer patients' prognosis. And the number of NK cell infiltrating in lung cancer tissue is closely related to the pathological types, size of the primary cancer, smoking history and prognosis of the patients with lung cancer. The expression of NK cells inhibitor receptors increased remarkably in tumor micro-environment, in opposite, the expression of NK cells activated receptors decrease magnificently.

CONCLUSIONS:

The survival time of lung cancer patient was positively related to NK cell infiltration degree in lung cancer. Thus, the down-regulation of NKG2D, Ly49I and the up-regulation of NKG2A may indicate immune tolerance mechanism and facilitate metastasis in tumor environment. Our research will offer more theory for clinical strategy about tumor immunotherapy.

PMID:
25299645
PMCID:
PMC4192363
DOI:
10.1371/journal.pone.0109976
[Indexed for MEDLINE]
Free PMC Article

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